Fig. 2

Hematoxylin and eosin (H&E) staining and immunohistochemical staining of CD34 and podoplanin (D2–40) in the nipple. a, d, g In a representative control case, only a few vessels are observed in the dermis (H&E staining (a), CD34 immunohistochemical staining (d), and podoplanin immunohistochemical staining (g)). b, e, h In a dermatitis case, a few vessels are observed with mild lymphocytic infiltration (H&E staining (b), CD34 immunohistochemical staining (e), and podoplanin immunohistochemical staining (h)). c, f, i In a mammary Paget disease case, Paget cells are observed in the epidermis, the number of vessels was markedly increased compared with the control and dermatitis cases, and lymphocytic infiltration is present in the dermis (H&E staining (c)). These vessels are predominantly CD34-positive (f), but several podoplanin-positive vessels are also seen (i). All magnification: × 100