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Table 1 Antibodies used in this study

From: Angiogenesis in mammary Paget disease: histopathological analyses of blood vessel density and angiogenic factors

Antibody Clone Host Source Dilution Incubation Pretreatment
Anti-CD34 QBEnd-10 Mouse Dako 1 / 100 Overnight / 4 °C or 60 min / RT (double staining) Microwave (CB)
Anti-podoplanin D2–40 Mouse Dako 1 / 50 30 min / RT No pretreatment
Anti-αSMA 1A4 Mouse Dako 1 / 100 30 min / RT No pretreatment
Anti-cytokeratin 7 OV-TL12/30 Mouse Dako 1 / 100 Overnight / 4 °C Electric pot (95 °C, TRS)
Anti-Ki-67 MIB-1 Mouse Dako 1 / 100 60 min / RT Pressure cooker (TRS)
Anti-bFGF 20B242.2 Mouse PeproTech 1 / 400 60 min / RT Electric pot (95 °C, TRS)
Anti-VEGFA SP28 Rabbit Abnova Ready to use 60 min / RT Electric pot (95 °C, CB)
  1. Abnova (Taipei, Taiwan); Dako (Glostrup, Demmark); PeproTech (Rochy Hill, NJ)
  2. bFGF Basic fibroblast growth factor, CB Citrate buffer, RT Room temperature, SMA Smooth muscle actin, TRS Target Retrieval Solution (Dako, pH 9), VEGFA Vascular endothelial growth factor-A