|
Antibody
|
Clone
|
Host
|
Source
|
Dilution
|
Incubation
|
Pretreatment
|
|---|
|
Anti-CD34
|
QBEnd-10
|
Mouse
|
Dako
|
1 / 100
|
Overnight / 4 °C or 60 min / RT (double staining)
|
Microwave (CB)
|
|
Anti-podoplanin
|
D2–40
|
Mouse
|
Dako
|
1 / 50
|
30 min / RT
|
No pretreatment
|
|
Anti-αSMA
|
1A4
|
Mouse
|
Dako
|
1 / 100
|
30 min / RT
|
No pretreatment
|
|
Anti-cytokeratin 7
|
OV-TL12/30
|
Mouse
|
Dako
|
1 / 100
|
Overnight / 4 °C
|
Electric pot (95 °C, TRS)
|
|
Anti-Ki-67
|
MIB-1
|
Mouse
|
Dako
|
1 / 100
|
60 min / RT
|
Pressure cooker (TRS)
|
|
Anti-bFGF
|
20B242.2
|
Mouse
|
PeproTech
|
1 / 400
|
60 min / RT
|
Electric pot (95 °C, TRS)
|
|
Anti-VEGFA
|
SP28
|
Rabbit
|
Abnova
|
Ready to use
|
60 min / RT
|
Electric pot (95 °C, CB)
|
- Abnova (Taipei, Taiwan); Dako (Glostrup, Demmark); PeproTech (Rochy Hill, NJ)
- bFGF Basic fibroblast growth factor, CB Citrate buffer, RT Room temperature, SMA Smooth muscle actin, TRS Target Retrieval Solution (Dako, pH 9), VEGFA Vascular endothelial growth factor-A
