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Table 1 Antibodies used in this study

From: Angiogenesis in mammary Paget disease: histopathological analyses of blood vessel density and angiogenic factors

AntibodyCloneHostSourceDilutionIncubationPretreatment
Anti-CD34QBEnd-10MouseDako1 / 100Overnight / 4 °C or 60 min / RT (double staining)Microwave (CB)
Anti-podoplaninD2–40MouseDako1 / 5030 min / RTNo pretreatment
Anti-αSMA1A4MouseDako1 / 10030 min / RTNo pretreatment
Anti-cytokeratin 7OV-TL12/30MouseDako1 / 100Overnight / 4 °CElectric pot (95 °C, TRS)
Anti-Ki-67MIB-1MouseDako1 / 10060 min / RTPressure cooker (TRS)
Anti-bFGF20B242.2MousePeproTech1 / 40060 min / RTElectric pot (95 °C, TRS)
Anti-VEGFASP28RabbitAbnovaReady to use60 min / RTElectric pot (95 °C, CB)
  1. Abnova (Taipei, Taiwan); Dako (Glostrup, Demmark); PeproTech (Rochy Hill, NJ)
  2. bFGF Basic fibroblast growth factor, CB Citrate buffer, RT Room temperature, SMA Smooth muscle actin, TRS Target Retrieval Solution (Dako, pH 9), VEGFA Vascular endothelial growth factor-A