Table 1 Antibodies used in this study
Antibody | Clone | Host | Source | Dilution | Incubation | Pretreatment |
|---|---|---|---|---|---|---|
Anti-CD34 | QBEnd-10 | Mouse | Dako | 1 / 100 | Overnight / 4 °C or 60 min / RT (double staining) | Microwave (CB) |
Anti-podoplanin | D2–40 | Mouse | Dako | 1 / 50 | 30 min / RT | No pretreatment |
Anti-αSMA | 1A4 | Mouse | Dako | 1 / 100 | 30 min / RT | No pretreatment |
Anti-cytokeratin 7 | OV-TL12/30 | Mouse | Dako | 1 / 100 | Overnight / 4 °C | Electric pot (95 °C, TRS) |
Anti-Ki-67 | MIB-1 | Mouse | Dako | 1 / 100 | 60 min / RT | Pressure cooker (TRS) |
Anti-bFGF | 20B242.2 | Mouse | PeproTech | 1 / 400 | 60 min / RT | Electric pot (95 °C, TRS) |
Anti-VEGFA | SP28 | Rabbit | Abnova | Ready to use | 60 min / RT | Electric pot (95 °C, CB) |