miR-541 serves as a prognostic biomarker of osteosarcoma and its regulatory effect on tumor cell proliferation, migration and invasion by targeting TGIF2

Table 2 Cox regression analysis in the patients with OS

Variables	Univariate analysis			Multivariate analysis
Variables	HR	95% CI	P value	HR	95% CI	P value
miR-541 low vs. high	2.612	1.523–5.845	0.004	2.599	1.312–5.012	0.006
Gender male vs. female	1.139	0.637–2.038	0.661	1.521	0.812–2.882	0.265
Age (years) ≥ 18 vs. < 18	1.124	0.640–1.975	0.684	1.234	0.575–2.127	0.713
Anatomical location tibia/femur vs. others	1.289	0.644–1.812	0.495	1.185	0.601–1.985	0.812
Tumor size (cm) ≥ 8 vs. < 8	1.520	0.867–2.665	0.144	1.321	0.685–2.254	0.451
Enneking stage IIB-III vs. I-IIA	2.412	1.428–5.625	0.008	2.212	1.198–4.114	0.016
Distant metastasis positive vs. negative	2.375	1.321–5.286	0.010	2.352	1.210–4.285	0.012
Histological subtype osteoblastic/chondroblastic vs. fibroblastic	1.485	0.856–2.458	0.128	1.521	0.721–2.386	0.324
Histological gradeTumor differentiation poor vs. well/moderate	1.968	1.089–4.996	0.039	1.985	1.085–3.891	0.046

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