Table 1 PCR primers used for Sanger sequencing
From: Pulmonary epithelial–myoepithelial carcinoma without AKT1, HRAS or PIK3CA mutations: a case report
Gene | Direction | Sequence (5′ to 3′) |
|---|---|---|
AKT1 exon 2 | Forward | AGGCACATCTGTCCTGGCAC |
Reverse | AAATCTGAATCCCGAGAGGCC | |
CTNNB1 exon 3 | Forward | TTTGATGGAGTTGGACATGG |
Reverse | AAAATCCCTGTTCCCACTCA | |
HRAS exon 2 | Forward | CAGGCCCCTGAGGAGCGATG |
Reverse | TTCGTCCACAAAATGGTTCT | |
HRAS exon 3 | Forward | TCCTGCAGGATTCCTACCGG |
Reverse | GGTTCACCTGTACTGGTGGGA | |
PIK3CA exon 9 | Forward | TGACAAAGAAGAGCTCAAAGC |
Reverse | TTAGCACTTACCTGTGACTCCA | |
PIK3CA exon 20 | Forward | TGATGACATTGCATACATTCG |
Reverse | TGTGTGGAAGATCCAATCCA |