Fig. 2

Immunohistochemistry (IHC) of the gastric carcinosarcoma showing H&E, AFP, and SALL4 immunostaining. a Tubular and solid growth patterns of adenocarcinoma with clear cytoplasm are visible; b The same area is positive for AFP by IHC; c Positivity for SALL4 staining is also shown; d indicates H&E staining showing a moderately differentiated adenocarcinomatous region with acidophilic cytoplasm; e this area indicates positivity of synaptophysin by IHC; f is also positive for chromogranin A; g is an H&E staining indicating proliferation of spindle cells with acidophilic cytoplasm; h this area is positive for SMA by IHC; i shows an H&E stained section indicating proliferation of atypical round cells; j: indicates positivity of desmin; and k MyoD1 by IHC; l: show a proliferative area of germ cell-like cells and proliferation of atypical “bare nuclei” cells is visible; m Immunohistochemically, the tumor is positive for SALL4. (scale bars in a–k: 50 μm and i, m: 20 μm)