Fig. 4

Flow cytometric and Southern blot analysis of gene rearrangements in video-assisted thoracoscopic surgery (VATS) specimen. a, b CD3 was focally expressed on the surface membrane (a) and expressed in the cytoplasm (b). c, d DNA from the lung tissue involved in lymphocyte infiltration after autologous hematopoietic stem cell transplantation (AutoHSCT) was digested with selected restriction enzymes (BamHI (Ba), EcoRI (Ec), and Hind III (Hi) for the T-cell receptor β (TCR β) gene and immunoglobulin heavy chain (IgH) gene). Control DNA was obtained from donated cord blood. DNA was probed with either a Cβ1 probe or a JH probe. TCR β gene rearrangements (arrows) were detected upon Ba, Ec, and Hi digestion (c). No rearrangement of IgH gene was found (d)