Fig. 2

a-b Representative images of microsatellite instability-low and the corresponding immunohistochemical results of mismatch repair proteins and histological findings. (a) BAT25 marker showing microsatellite instability and lack of immunohistochemistry of MLH1, MSH2, MSH6, and PMS2 are noted in the poorly-differentiated squamous cell carcinoma with high tumor infiltrating lymphocytes. b BAT25 marker showing microsatellite instability and lack of immunohistochemistry of MSH2 and MSH6 are observed in the well-differentiated squamous cell carcinoma with low tumor infiltrating lymphocytes. c Real-time peptide nucleic acid probe-based fluorescence melting curve analysis reveals the HPV68 genotyping. A fungating mass (3.5 × 1.8 × 0.8 cm) is observed in the lower portion of esophagus. Poorly-differentiated esophageal squamous cell carcinoma with discohesive tumor cells