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Fig. 5 | Diagnostic Pathology

Fig. 5

From: Circ_0001421 facilitates glycolysis and lung cancer development by regulating miR-4677-3p/CDCA3

Fig. 5

CDCA3 was a target gene of miR-4677-3p in LC. a The predicted binding sites between miR-4677-3p and CDCA3 were shown. b The relationship between miR-4677-3p and CDCA3 in H1299 and A549 cells was analyzed by dual-luciferase reporter assay. c-d The protein of CDCA3 in LC tissues and cells versus that in normal tissues and cells was examined by western blot. e The correlation between miR-4677-3p and CDCA3 expression was appraised in LC tissues through Pearson coefficient analysis. f The correlation between miR-4677-3p and circ_0001421 level was appraised in LC tissues through Pearson coefficient analysis. g The protein of CDCA3 in H1299 and A549 cells transfected with miR-4677-3p or miR-NC was measured by western blot. h-i The protein of CDCA3 in H1299 and A549 cells transfected with si-NC, si-circ_0001421, si-circ_0001421 + anti-miR-NC or si-circ_0001421 + anti-miR-4677-3p was determined by western blot. *P < 0.05

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