Fig. 5

EIF5A2 is a target of miR-218-5p. a The binding sites of miR-218-5p on EIF5A2. b, c Dual-luciferase reporter assay in Huh7/DOX and HCCLM3/DOX cells co-transfected with the reporter plasmid and the indicated miRNAs. d-i mRNA and protein levels analysis of EIF5A2 in HCC tissues, DOX-resistant tissues and matched normal tissues (d, e), normal hepatocyte THLE-2 and HCC cell lines (Hep3B, Huh7 and HCCLM3) (f, g), as well as DOX-resistant HCC cell lines and parental HCC cells (h, i) using qRT-PCR and western blot. j Correlation analysis between EIF5A2 and miR-218-5p expression. k, l Levels analysis of EIF5A2 expression in Huh7/DOX and HCCLM3/DOX cells transfected with miR-NC or miR-218-5p using qRT-PCR and western blot. *P < 0.05