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Fig. 6 | Diagnostic Pathology

Fig. 6

From: Circ_0003998 enhances doxorubicin resistance in hepatocellular carcinoma by regulating miR-218-5p/EIF5A2 pathway

Fig. 6

MiR-218-5p alleviates DOX resistance in HCC cells through modulating EIF5A2 expression. Huh7/DOX and HCCLM3/DOX cells were transfected with miR-NC, miR-218-5p, miR-218-5p + pcDNA-NC, or miR-218-5p + pcDNA-EIF5A2. After transfection, a, b qRT-PCR and western blot analysis of EIF5A2 levels in resistant cells. c, d CCK-8 analysis of resistant cell viability in combination with increasing concentrations of DOX (0, 0.0625, 0.125, 0.25, 0.5, or 1 μg/mL); e colony formation analysis of resistant cell viability with 0.5 μg/mL DOX; f, g transwell analysis of the abilities of migration and invasion in resistant cells; h, i level analysis of MMP9, Vimentin, N-cadherin, and E-cadherin in resistant cells with western blot. *P < 0.05

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Diagnostic Pathology

ISSN: 1746-1596

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