Table 1 Primers used for the Sanger sequencing
From: An NRAS mutation in primary malignant melanoma of the lung: a case report
Gene | Exons | 5′ → 3′ Sequence | Tm | Length |
|---|---|---|---|---|
BRAF | EX15 | F: TCATAATGCTTGCTCTGATAGG | 60 | 224 |
R: GGCCAAAAATTTAATCAGTGG | ||||
NRAS | EX2 | F: GAAAGCTTTAAAGTACTGTAGATGTGG | 60 | 247 |
R: AGATGATCCGACAAGTGAGAGA | ||||
NRAS | EX3 | F: CCCCTTACCCTCCACACC | 60 | 243 |
R: CACAAAGATCATCCTTTCAGAGAA |