|
Patients and tumor characteristics
|
Total samples N (%)
|
H-score (cut off = 149) N (%) Cytoplasmic TWIST1 expression
|
P-value
|
H-score (cut off = 172) N (%) Nuclear TWIST1 expression
|
P-value
|
|---|
|
Low (≤ 149)
|
High (> 149)
|
Low (≤ 172)
|
High (> 172)
|
|---|
|
Number of CRC samples
|
223
|
113 (50.7)
|
110 (49.3)
|
87 (39.0)
|
136 (61.0)
|
|---|
|
Mean age, years (Range)
|
59 (20–91)
| | |
0.159
| | |
0.262
|
|
≤ Mean age
|
110 (49.3)
|
61 (54.0)
|
49 (44.5)
| |
47 (54.0)
|
63 (46.3)
| |
|
> Mean age
|
113 (50.7)
|
52 (46.0)
|
61 (55.5)
| |
40 (46.0)
|
73 (53.7)
| |
|
Gender
| | | |
0.095
| | |
0.129
|
|
Male
|
114 (51.1)
|
64 (56.6)
|
50 (45.5)
| |
50 (57.5)
|
64 (47.1)
| |
|
Female
|
109 (48.9)
|
49 (43.4)
|
60 (54.5)
| |
37 (42.5)
|
72 (52.9)
| |
|
(Male/Female)
|
1.04
|
1.30
|
0.83
| |
1.35
|
0.88
| |
|
Mean tumor size (cm)
|
5
| | |
0.389
| | |
0.522
|
|
≤ Mean
|
156 (70.0)
|
82 (72.6)
|
74 (67.3)
| |
63 (72.4)
|
93 (68.4)
| |
|
> Mean
|
67 (30.0)
|
31 (27.4)
|
36 (32.7)
| |
24 (27.6)
|
43 (31.6)
| |
|
Tumor differentiation
| | | |
0.549
| | |
0.405
|
|
Well
|
103 (46.2)
|
53 (46.9)
|
50 (45.5)
| |
45 (51.7)
|
58 (42.6)
| |
|
Moderate
|
104 (46.6)
|
50 (44.2)
|
54 (49.1)
| |
36 (41.4)
|
68 (50.0)
| |
|
Poor
|
16 (7.2)
|
10 (8.8)
|
6 (5.5)
| |
6 (6.9)
|
10 (7.4)
| |
|
TNM stage
| | | |
0.932
| | |
0.831
|
|
I
|
40 (17.9)
|
19 (16.8)
|
21 (19.1)
| |
16 (18.4)
|
24 (17.6)
| |
|
II
|
98 (43.9)
|
52 (46.0)
|
46 (41.8)
| |
40 (46.0)
|
58 (42.6)
| |
|
III
|
79 (35.4)
|
39 (34.5)
|
40 (36.4)
| |
28 (32.2)
|
51 (37.5)
| |
|
IV
|
6 (2.7)
|
3 (2.7)
|
3 (2.7)
| |
3 (3.4)
|
3 (2.2)
| |
|
Vascular invasion (VI)
| | | |
0.783
| | |
0.445
|
|
Present
|
36 (16.1)
|
19 (16.8)
|
17 (15.5)
| |
12 (13.8)
|
24 (17.6)
| |
|
Absent
|
187 (83.9)
|
94 (83.2)
|
93 (84.5)
| |
75 (86.2)
|
112 (82.4)
| |
|
Lymph node invasion (LNI)
| | | |
0.878
| | |
0.571
|
|
Involved
|
82 (36.8)
|
41 (36.3)
|
41 (37.3)
| |
30 (34.5)
|
52 (38.2)
| |
|
None
|
141 (63.2)
|
72 (63.7)
|
69 (62.7)
| |
57 (65.5)
|
84 (61.8)
| |
|
Neural invasion (NI)
| | | |
0.697
| | |
0.653
|
|
Involved
|
47 (21.1)
|
25 (22.1)
|
22 (20.0)
| |
17 (19.5)
|
30 (22.1)
| |
|
None
|
176 (78.9)
|
88 (77.9)
|
88 (80.0)
| |
70 (80.5)
|
106 (77.9)
| |
|
Distant metastasis
| | | |
0.234
| | |
0.045
|
|
Present
|
37 (16.6)
|
15 (13.3)
|
22 (20.0)
| |
8 (9.2)
|
29 (21.3)
| |
|
Absent
|
100 (44.8)
|
52 (46.0)
|
48 (43.6)
| |
40 (46.0)
|
60 (44.1)
| |
|
Not identified
|
86 (38.6)
|
46 (40.7)
|
40 (36.4)
| |
39 (44.8)
|
47 (34.6)
| |
|
Tumor recurrence
| | | |
0.568
| | |
0.149
|
|
Yes
|
42 (18.8)
|
19 (16.8)
|
23 (20.9)
| |
11 (12.7)
|
31 (22.8)
| |
|
No
|
95 (42.6)
|
48 (42.5)
|
47 (42.7)
| |
37 (42.5)
|
58 (42.6)
| |
|
Not identified
|
86 (38.6)
|
46 (40.7)
|
40 (36.4)
| |
39 (44.8)
|
47 (34.6)
| |
- H-score indicates histological score
- Values in bold are statistically significant
