Table 2 The primers sequences in qRT-PCR
From: Let-7a-5p regulates the inflammatory response in chronic rhinosinusitis with nasal polyps
Gene | Primers sequences | |
|---|---|---|
let-7a-5p | Forward | 5′-GGGAGAAGTCCGCTGGTGTTG-3′ |
let-7a-5p | Reverse | 5′-CTGATCTCCTTGTTCAAGTTCA-3′ |
U6 | Forward | 5′-CTCGCTTCGGCAGCACA-3′ |
U6 | Reverse | 5′-AACGCTTCACGAATTTGCGT-3′ |
TNF-α | Forward | 5′-CACCATGAGCACTGAAAGCA-3′ |
TNF-α | Reverse | 5′-GCTCTTGATGGCAGAGAGGAG-3′ |
IL-1β | Forward | 5′-TTCGAGGCACAAGGCACAAC-3′ |
IL-1β | Reverse | 5′-CTGGAAGGAGCACTTCATCTGT-3′ |
IL-6 | Forward | 5′-ACCCCCAGGAGAAGATTCCA-3′ |
IL-6 | Reverse | 5′-GTCTTCCCCCACACCAAGTT-3′ |
GAPDH | Forward | 5′-GCCACAACGACCCCTTCATG-3′ |
GAPDH | Reverse | 5′-TGCCAGTGAGCTTCCCGTTC-3′ |