Fig. 2

Downregulation of TUG1 inhibited proliferation, migration, invasion, and induced apoptosis of CRC cells. a The expression of TUG1 in CRC cell lines (LoVo and HCT116) and human normal intestinal mucous cell line (CCC-HIE-2) was evaluated by qRT-PCR. b The level of TUG1 in CRC cells transfected with si-NC or si-TUG1 was determined by qRT-PCR. c, d MTT assay was used to detect cell viability of LoVo and HCT116 cells transfected with si-NC or si-TUG1 at different time points. e Flow cytometry was used to detect apoptosis of transfected CRC cells at 48 h after transfection. f, g Transwell migration and invasion assays were performed, and the cell numbers of migration or invasion were calculated. *P < 0.05