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Fig. 4 | Diagnostic Pathology

Fig. 4

From: Long noncoding RNA TUG1 regulates the progression of colorectal cancer through miR-542-3p/TRIB2 axis and Wnt/β-catenin pathway

Fig. 4

Overexpression of TRIB2 rescued the effects of TUG1 silencing-mediated on proliferation, migration, invasion and apoptosis of CRC cells. a, b The expression of TRIB2 in CRC cells transfected with si-TUG1 or si-TUG1 + pcDNA-TRIB2, as well as corresponding controls, was detected by qRT-PCR. c, d The protein level of TRIB2 in transfected CRC cells was measured by western blot. e, f Viability of transfected CRC cells was evaluated by MTT assay at different time points. g, h Apoptosis of transfected CRC cells was checked by flow cytometry. i, j Transwell chamber without Matrigel was hired to assess migration of transfected CRC cells. k, l Transwell chamber pre-coated with Matrigel was used to check the invasion of transfected CRC cells. *P < 0.05

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