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Fig. 5 | Diagnostic Pathology

Fig. 5

From: Long noncoding RNA TUG1 regulates the progression of colorectal cancer through miR-542-3p/TRIB2 axis and Wnt/β-catenin pathway

Fig. 5

TUG1 regulated TRIB2 by interacting with miR-542-3p in CRC cells. a The putative binding sites between TUG1 and miR-542-3p were predicted by starBase. b, c The luciferase activity of CRC cells co-transfected with miR-542-3p and WT-TUG1 or MUT-TUG1 was checked. d, e The level of miR-542-3p in Lovo and HCT116 cells transfected with si-TUG1 or pcDNA-TUG1, as well as matched controls was determined by qRT-PCR. And the specificity of amplification of miR-542-3p primer pair was verified by agarose gel visualization. Correlation between miR-542-3p expression and TUG1 expression was assessed by Pearson’s correlation coefficient. g The putative binding sites between miR-542-3p and TRIB2 were forecasted by starBase. h, i The Dual-luciferase reporter assay was performed to confirm the interaction between miR-542-3p and TRIB2. j, k The protein level of TRIB2 in CRC cells transfected with miR-542-3p or miR-542-3p + pcDNA-TUG1, as well as matched controls, was measured by western blot. l Pearson’s correlation coefficient for the relationship between miR-542-3p expression and TRIB2 expression. *P < 0.05

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