Table 1 Summaries of FISH cut-off and scanning profile
From: Optimized workflow for digitalized FISH analysis in pathology
A) Break apart probes with comparison of cut off values from the literature. ° dual colour dual fusion | |||
Probe | Provider | Cut off (%) | Cut in the literature (%) |
BCL-2 | Zytovision | 4 | 10 |
BCL-6 | Zytovision | 4 | 8 |
CCND1 | Zytovision | 4 | 1.3 |
COL-A1 | Zytovision | 5 | 5 ° |
EWSR1 | Vysis/Abbott | 7 | 10 |
FUS | Vysis/Abbott | 3 | NA |
C-MYC | Vysis/Abott | 5 | 9 |
USP-6 | Vysis/Abbott | 3 | 20 |
B) Comparison of scanning time, file size and FOV (fields of fiew) scanned with the two profiles in 42 diagnostic cases | |||
Profile | All (n = 42) | High (n = 26) | Low (n = 16) |
scanning time in min | |||
Min | 1 | 3 | 1 |
Max | 1620 | 1620 | 72 |
Mean, Total | 87 | 159 | 15 |
Mean, <10’000 FOV | 34 | 57 | 11 |
file size | |||
Min | 17 | 17 | 30 |
Max | 7620 | 7620 | 940 |
Mean, total | 794 | 1129 | 458 |
Mean, <10’000 FOV | 468 | 607 | 328 |
FOV | |||
Min | 105 | 105 | 120 |
Max | 58830 | 58830 | 10620 |
Mean, total | 3840 | 5971 | 1708 |
Mean, <10’000 FOV | 1713 | 2311 | 1114 |