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Table 1 Summaries of FISH cut-off and scanning profile

From: Optimized workflow for digitalized FISH analysis in pathology

A) Break apart probes with comparison of cut off values from the literature. ° dual colour dual fusion
Probe Provider Cut off (%) Cut in the literature (%)
BCL-2 Zytovision 4 10
BCL-6 Zytovision 4 8
CCND1 Zytovision 4 1.3
COL-A1 Zytovision 5 5 °
EWSR1 Vysis/Abbott 7 10
FUS Vysis/Abbott 3 NA
C-MYC Vysis/Abott 5 9
USP-6 Vysis/Abbott 3 20
B) Comparison of scanning time, file size and FOV (fields of fiew) scanned with the two profiles in 42 diagnostic cases
Profile All (n = 42) High (n = 26) Low (n = 16)
scanning time in min
Min 1 3 1
Max 1620 1620 72
Mean, Total 87 159 15
Mean, <10’000 FOV 34 57 11
file size
Min 17 17 30
Max 7620 7620 940
Mean, total 794 1129 458
Mean, <10’000 FOV 468 607 328
FOV
Min 105 105 120
Max 58830 58830 10620
Mean, total 3840 5971 1708
Mean, <10’000 FOV 1713 2311 1114