Fig. 4

ASMTL-AS1 regulates the miR-1228-3p/SOX17/β-catenin axis. a, b. The binding site between SOX17 3`-UTR and miR-1228-3p, followed by mutation and luciferase reporter assay. c, d. qRT-PCR analysis of SOX17 mRNA level after alteration of miR-1228-3p expression. e. Western blotting testing SOX17 and β-catenin protein levels in ASMTL-AS1-overexpressing cells transfected with miR-1228-3p mimics. f. qRT-PCR analysis of β-catenin targets in ASMTL-AS1-overexpressing cells transfected with miR-1228-3p mimics. g, h. CCK-8 and Transwell assays respectively testing cell viability and invasion in ASMTL-AS1- overexpressing cells transfected with miR-1228-3p mimics, SOX17 siRNA or treated with LiCl, a specific activator of Wnt/β-catenin pathway. ^*P < 0.05, ^**P < 0.01