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Fig. 3 | Diagnostic Pathology

Fig. 3

From: LncRNA GAS5 modulates the progression of non-small cell lung cancer through repressing miR-221-3p and up-regulating IRF2

Fig. 3

miR-221-3p was validated to be one of the downstream targets of GAS5. a The binding sequence between GAS5 and miR-221-3p was predicted by the StarBase database. b Verification of the binding site between miR-221-3p and GAS5 was conducted by dual-luciferase reporter gene experiment. c The effects of overexpression and knockdown of GAS5 on miR-221-3p expression in NSCLC cells were detected by qPCR. d The correlation between GAS5 and miR-221-3p expressions in NSCLC tissues. e MiR-221-3p expression in NSCLC tissues and paracancerous tissues was detected by qPCR. f MiR-221-3p expression in human normal lung epithelial cell line and each NSCLC cell line was detected by qPCR. g Transfection efficiency of miR-221-3p mimics and miR-221-3p inhibitors was verified using PCR. *** represents P < 0.001

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