Fig. 2

Amplification curves of EGFR T790M case 4. Idylla Explore analysis software amplification curves of C (which includes the curves containing the T790M mutation) are presented for analysis of EGFR T790M case 4 using A 1 slide, B 2 slides, C) 3 slides. Relevant curves: brown curve is the T790M-specific amplification curve. Black curve a sample processing control curve that reflects the amount of amplifiable DNA in the sample. Green (Del12) and pink (Del18/21b/24) curves are used for the detection of specific EGFR deletions, both deletion types are not present in this specimen