Fig. 2

MiR-1297 binds to DUXAP8 and is negatively mediated by DUXAP8. A-B. The location of DUXAP8 in caSki and HeLa cells was detected by subcellular fractionation and FISH assays. C. RT-qPCR analysis was performed to detect the expression of candidate miRNAs in CC cells with or without DUXAP8 depletion. D. Putative and mutant binding sites between DUXAP8 and miR-1297. E. The expression of miR-1297 in CC cell line and control cells was determined by RT-qPCR. F. Luciferase reporter assays showed that miR-1297 overexpression could impair the luciferase activity of DUXAP8-WT. G. RNA pull down showed that DUXAP8 was preferentially enriched by biotin-labeled miR-1297-WT. H. The expression of miR-1297 was evaluated by RT-qPCR in two CC cells after transfecting with miR-1297 inhibitor. I-N. Rescue experiments were implemented in HeLa cells to elucidate the influence of miR-1297 inhibitor on the cellular activities of DUXAP8-silenced CC cells. **P < 0.01