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Fig. 4 | Diagnostic Pathology

Fig. 4

From: Long non-coding RNA DUXAP8 elevates RCN2 expression and facilitates cell malignant behaviors and angiogenesis in cervical cancer via sponging miR-1297

Fig. 4

LncRNA DUXAP8 regulates CC cellular behaviors via up-regulating RCN2 by miR-1297. A. The expression of RCN2 in HeLa cells after transfecting with pcDNA3.1/RCN2 was detected by RT-qPCR. B-C. Colony formation and EdU assays were conducted to explore the proliferation of HeLa cells transfected with NC mimics, miR-1297 mimics or miR-1297 mimics + pcDNA3.1/RCN2. D. Flow cytometry analysis was performed to assess the rate of apoptotic cells under different conditions. E-G. Transwell and tube formation assays were conducted to determine cell migration, invasion and angiogenesis under diverse contexts. H-M. Rescue functional experiments were performed among sh-NC, sh-DUXAP8#1 and sh-DUXAP8#1 + pcDNA3.1/RCN2 groups to explore the effects of RCN2 overexpression on DUXAP8 inhibition-mediated function in HeLa cells. **P < 0.01

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Diagnostic Pathology

ISSN: 1746-1596

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