Table 1 Antibodies used in the present study
Antigen | Clone | Source | Equipment | Antigen retrieval |
|---|---|---|---|---|
PON | (P) | Abcam PLC (Cambridge, UK) | L | ER1 (30 min) |
Galectin-3 | 9C4 | Leica Biosystems (Bannockburn, IL, USA) | L | ER1 (30 min) |
CK19 | ROL108 | DakoCytomation (Carpinteria, CA, USA) | L | ER2 (30 min) |
HBME-1 | HBME-1 | DakoCytomation (Carpinteria, CA, USA) | L | ER2 (30 min) |
Cyclin D1 | SP4 | Invitrogen (Carlsbad, CA, USA) | L | ER2 (20 min) |
Ki-67 | MIB1 | DakoCytomation (Carpinteria, CA, USA) | R | CC1 (64 min) |