Fig. 3

Knockdown of CircRNA NRIP1 inhibited cell proliferation, invasion and induced apoptosis of TPC1 and IHH-4 cells. A Interference efficiency measurement in the TPC1 and IHH-4 cells transfected with negative sh-NC or sh-NRIP1 plasmids via qRT-PCR. B Proliferation of TPC1 and IHH-4 cells transfected with sh-NRIP1 and sh-NC for indicated times and the proliferative capacity was detected using MTT assays. C TPC1 and IHH-4 cells treated with sh-NRIP1 or not were experienced with Annexin V-PE/7-AAD coloration at 48 h and apoptosis was calculated via flow cytometry. D The effect of CircRNA NRIP1 knockdown on cell invasion function was performed via transwell assay at 24 h, and the representative pictures of cells migration results were shown. Magnification ×100. *P < 0.05