Fig. 3
From: Establishment of multiplex RT-PCR to detect fusion genes for the diagnosis of Ewing sarcoma
Multiplex RT–PCR in tumor samples. Multiplex (top and middle panels) and control (bottom panel) RT–PCR were performed in six tumor samples. Lane M: Trackit 100-bp ladder marker (top panel) or Trackit 1-kbp plus ladder (middle and bottom panels). Molecular sizes are indicated in the right with yellow arrowheads (a) and red arrowheads (b), respectively.; lane 1: NCR-EW2 cDNA for the positive control; lane 2: tumor 1; lane 3: tumor 2; lane 4: tumor 3; lane 5: tumor 4; lane 6: tumor 5; lane 7: tumor 6; lane 8: NRS-1 cDNA for the negative control; lane 9: no template control