Fig. 2

LGR5 expression at the site of fat invasion. Representative hematoxylin and eosin (HE)-stained images of low histological grade (HG) (A and B) and high HG (C) tissues. In low HG tissues, LGR5 expression was relatively easy to identify (D and E). Detailed images of LGR5-positive dots (arrows) are shown in the insert image in D and E. In high HG, low LGR5 expression was identified (F). A detailed image of a LGR5-positive dot (arrow) is shown in the insert image in F. In low HG tissues, E-cadherin expression was identified (G and H). In high HG, E-cadherin expression was not identified (I). In low HG tissues, vimentin expression was not identified (J and K). In high HG, vimentin expression was identified (L). (A, B, and C: HE staining, 40× magnification; D, E, and F: LGR5 RNAscope, 40× magnification [insert image 60× magnification]; G, H, and I: E-cadherin, 40× magnification; J, K, and L: vimentin, 40× magnification)