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Table 3 Summary data for sample stability, longevity & reagent reproducibility. RT = reverse transcription, OA = open array, MM = master mix

From: Analytical validity of DecisionDx-SCC, a gene expression profile test to identify risk of metastasis in cutaneous squamous cell carcinoma (SCC) patients

Additional Characteristics Sample Overall Class Call Concordance Acceptable
Longevity, RNA Stability n = 23 96% (22/23) ≥ 90%
cDNA stability n = 15 RT hold 100% (15/15) ≥ 90%
Lot to Lot Reagents Reproducibility n = 10 Extraction 90% (9/10) ≥ 90%
n = 11 RT Kit & RNase 91% (10/11) ≥ 90%
n = 5 Pooled Assay & Pre-amplification MM 100% (5/5) ≥ 90%
n = 8 OA & OA MM 100% (8/8) ≥ 90%