Tumor Area Positivity (TAP) score of programmed death-ligand 1 (PD-L1): a novel visual estimation method for combined tumor cell and immune cell scoring

Table 2 TAP scoring precision for determining PD-L1 expression level in the internal study (including borderline cases)

Study	Statistics	Agreement	Results^a
Between-reader	APA %	99.3	Pass
	n/N	296/298
	95% CI	98.0–100.0
	ANA %	99.3	Pass
	n/N	300/302
	95% CI	98.0–100.0
	OPA %	99.3	Pass
	n/N	298/300
	95% CI	98.0–100.0
Within-reader	APA %	99.0	Pass
	n/N	296/299
	95% CI	98.0–100.0
	ANA %	99.0	Pass
	n/N	298/301
	95% CI	98.0–100.0
	OPA %	99.0	Pass
	n/N	297/300
	95% CI	98.0–100.0
Background	OPA %	100.0	Pass
	n/N	600/600
	95% CI	99.4–100.0

Counts indicate the number of pairwise comparisons and do not represent the number of unique cases
ANA Average negative agreement, APA Average positive agreement, CI Confidence interval, OPA Overall percent agreement, PD-L1 Programmed death-ligand 1, TAP Tumor Area Positivity
^aThe acceptance criteria for between-reader precision were ≥ 85% ANA and APA; the acceptance criteria for within-reader precision were ≥ 90% OPA, and ≥ 85% ANA and APA. The acceptance criterion for background staining was that the assay must produce acceptable levels of non-specific staining on ULTRA instruments in at least 90% of samples

ISSN: 1746-1596