Table 1 Studies characteristics and the impact of CAIX on recurrence free survival
Author(s) | Country | Patients (n) | Median follow up (months) | Cancer death n (%) | Subtype | Antibody for IHC/ dilution | Scoring method | High CAIX expression n (%) | Score range and location | Definition of positive | Tumour stage | Multivariate variables | Hazard ratio (95% CI) | p-value | HR estimation |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Beketic-Oreskovic et al. 2011 [23] | Croatia | 40 | 55.8 | 24 (60) | Mixed BC | NA 1:100 | Percentage and intensity | 24 (60) | Score1-3 Membranous and cytoplasmic | Score > 52.5 | NA | Necrosis, tumour size, LN, histological grade and CAIX expression | MV analysis 3.99 (1.38–11.59) | 0.011 | Reported in text |
Lou et al. 2011 [19] | Canada | 3,630 | 126 | NA | Mixed BC | M75 1:50 | Absent or present | 566 (16) | 0–1 Location not stated | Score ≥ 1 | NA | NA | UV analysis 1.4 |  < 10` 17 | Reported in text |
Lancashire et al. 2010 [48] | United Kingdom | 244 | 67 | NA | Mixed BC (n = 160) | Abcam 15,086 1:2,500 | Absent or present | 29 (18) | 0–1 Membranous | Score ≥ 1 | NA | NA | UV analysis NA | 0.097 | Reported in text |
Crabb et al. 2008 [49] | Canada | 313 | NA | NA | Mixed BC | M75 1:50 | Absent or present | 47 (15) | 0–1 Location not stated | Score ≥ 1 | NA | ER, PR, Her-2 status, EGFR, Ki67, p53, CK5/6 and CAIX expression | MV analysis 1.67 (1.06–2.64) | 0.03 | Reported in text |
Trastour et al. 2007 [16] | France | 132 | 138 | 20 (15) | Mixed BC | MN75 1:10,000 | Percentage | 38 (29) | 0–100 Membranous |  > 1% | NA | Tumour size, LN status, tumour grade, HIF-1a and CAIX expression | MV analysis 2.7 (1.2–6.1) | 0.01 | Reported in text |
Brennan et al. 2006 [50] | Sweden | 400 | 166.8 | NA | Mixed BC LN + ve (n = 104) premenopausal | M75 1:2000 | Absent or present | 42 (11) | 0–1 Membranous | Score ≥ 1 | II | NA | UV analysis NA | 0.032 | Reported in text |
Chia et al. 2001 [13] | United Kingdom | 103 | 74.4 | 32 (31) | Mixed BC | M75 1:50 | Percentage and intensity | 49 (48) | Score 0–300 Membranous | Score ≥ 1 | NA | LN status, grade, size, ER, necrosis and CAIX expression | MV analysis 2.13 | 0.06 | Reported in text |