Figure 1From: Development of automated brightfield double In Situ hybridization (BDISH) application for HER2 g ene and chromosome 17 centromere (CEN 17) for breast carcinomas and an assay performance comparison to manual dual color HER2 fluorescence In Situ hybridization (FISH)Brightfield double in situ hybridization (BDISH) signal detection scheme with a sequential in situ hybridization method. HER2 gene signal was detected with a DNP-labeled nick translated DNA probe hybridization followed by silver signal detection system (silver acetate, hydroquinone, and H2O2 reaction). Then, chromosome 17 centromere (CEN 17) signal was detected with a DNP-labeled CEN 17 oligoprobe hybridization followed by fast red and naphthol phosphate reaction signal detection system.Back to article page