Figure 1

Up-regulation of the expression of TLR2, TLR3, TLR4, TLR7, TLR9, NOD1, and NOD2 in human PBMCs in response to anti-PR3 Abs. PBMCs were stimulated with murine anti-human PR3 Abs (1 μg/ml, filled graphs) or an isotype-matched control IgG (1 μg/ml, bold line). After 6 h of incubation, the expression of cell-surface TLR2 and TLR4 and intracellular TLR3, TLR7, TLR9, NOD1, and NOD2, was assessed by flow cytometry. The thin lined curve is the staining with a control Ab. The results presented are representative of four different experiments.