Table 1 erb B antibodies used
From: Overexpression of c-erb B2 is a negative prognostic factor in anaplastic astrocytomas
Antibody | Source | Type | Clone | Reactant | Positive control | Dilution | Incubation time and temperature |
|---|---|---|---|---|---|---|---|
NCL-EGFR-384 | Novocastra, Newcastle-upon-Tyne, UK | Monoclonal | EGFR.25 | EGFR (c-erb B1 protein) (internal domain) | Human skin | 1:100 | 60 min. at room temperature |
NCL-EGFR | Novocastra | Monoclonal | EGFR.113 | EGFR (c-erb B1 protein) (external domain) | Human skin | 1:10 | 60 min. at room temperature |
c-erb B-2 | Novocastra | Monoclonal | CB11 | c-erb B2 protein (internal domain) | SKBR-3 breast cancer cell line | 1:40 | 60 min. at room temperature |
c-erb B-2 | Immunotech, Marseille, France | Monoclonal | 3B5 | c-erb B2 protein (internal domain) | SKBR-3 breast cancer cell line | Ready to use | 60 min. at room temperature |
c-erb B-2 | Novocastra | Monoclonal | 5A2 | c-erb B2 protein (internal domain) | SKBR-3 breast cancer cell line | 1:50 | 60 min. at room temperature |
NCL-c-erb B-3 | Novocastra | Monoclonal | RTJ1 | c-erb B-3 protein | Normal kidney | 1:10 | Over night at 4°C |
c-erb B-4/HER4 | NeoMarkers (Fremont, CA, USA) | Monoclonal | HFR-1 | c-erb B-4 protein | Breast carcinoma | 1:10 | Over night at 4°C |