Table 2 Immunohistochemical and Tissue of Origin Test results
Immunohistochemistry Results | Tissue of Origin (TOO) Test results | |||||||
|---|---|---|---|---|---|---|---|---|
Case No. | Negative | Positive | Suspected tissues based on clinicopathology | TOO result | TOO SS | Rule out tissues | Consistent | Management Value |
1 | BRST-2, CK20, TTF-1, ER, PR, Her2/neu | CK7 | Pancreas, Breast, Upper GI | Indeterminate | 22.1 | 8 | -- | -- |
2 | PSA | CK20, CK7, CDX2* | Kidney | Colorectal | 83.7 | 14 | No* | Yes |
3 | BRST-2, CK20, TTF-1, ER, PR, Her2/neu | CK20, CK7, WT1 | Ovary or Breast | Ovary | 87.6 | 14 | Yes | Yes |
4 | chromogranin, synaptophysin, calretinin, TTF-1, PR | CK20, CK7, CDX-2, CK5/6, MOC31, ER, CEA | GI Primary, Colorectal | Colorectal | 81.8 | 13 | Yes | Yes |
5 | CDX-2, CD31, CK20, Melan A, S-100, TTF-1 | AE1/AE3, CK7 | Renal cell carcinoma | Indeterminate | 24.7 | 11 | -- | -- |
6 | Thyroglobulin | TTF-1 | Lung, Thyroid | Breast | 61.2 | 12 | No* | Yes |
7 | CK20, PSA, TTF-1 | CK7, AE1/AE3, keratin 903, p63 | Lung | Indeterminate | 23.6 | 8 | -- | |
8 | BRST-2, CD10, CD45, C-kit | CDX-2, CAM5.2, CK20, CK7, Keratin | Colorectal | Colorectal | 64.1 | 12 | Yes | Yes |
9 | CK20, CK7, TTF-1, ER, PR | Colorectal, Ovary | Lung | 35.5 | 10 | No* | Yes | |
10 | CDX-2*, CK20, CK7 | Colorectal, Ovary | Colorectal | 71.2 | 13 | Yes | Yes | |
11 | ER, PR, Her2/neu, BRST-2, CDX-2, CK20 | CK7, p53, WT1 | Breast, Ovary | Ovarian | 86.6 | 14 | Yes | Yes |
12 | chromogranin, synaptophysin, surfactant, TTF, CK20, LCA, CA-125, Her2/neu | ER, PR, monoclonal CEA, focal CK7 | Breast, Ovary, Uterine | Breast | 96.4 | 14 | Yes | Yes |
13 | Her2/neu, PR | estrogen receptor (10% cells staining at 1+) | Breast | Breast | 95 | 14 | Yes | Yes |
14 | HMB45, Tyrosinase, Melan-A, RCC, ER | CK7, AE1/AE3, CAM 5.2, TTF, S100, surfactant protein, CK20; weak CDX-2 | Lung | Ovarian | 87.5 | 13 | No* | Yes |
15 | EGF-R | Lung | Lung | 66.9 | 12 | Yes | Yes | |
16 | LeuM1, S100 | PLAP, CA-125, monoclonal CEA | Pancreas, Ovary | Pancreas | 39.5 | 11 | Yes | Yes |
17 | PSA, neuroendocrine markers, S100 | mucicarmine, CEA, cytokeratin | Colon, Prostate, Stomach, Lung | Pancreas | 37.3 | 10 | No* | Yes |
18 | PSA, TTF, calretinin, thrombomodulin, P63 | CK7, CK20, monoclonal CEA, CDX-2 | Esophagus, Stomach, Pancreas, Biliary | Breast | 36.1 | 10 | No* | Yes |
19 | smooth myo actin, desmin, S100, HMB-45, CD34, chromogranin, CK7 | focal C-kit, focal synaptophysin, focal CK20, mucicarmine, PAS | Colon | Colorectal | 31.5 | 8 | Yes | Yes |
20 | Not available | Not available | None | Indeterminate | 24 | 9 | -- | -- |
21 | mucicarmine, HepPar, alpha-fetoprotein, synaptophysin | CEA monoclonal/polyclonal, Cam-5.2, AE1 | Liver, Cholangiocarcinoma | Indeterminate | 20.7 | 9 | -- | -- |