Antibody | Pretreatment | Incubation | Detection | Dilution | Company | |
---|---|---|---|---|---|---|
AMACR | Monoclonal | 98°C Hot water, pH 9.0, | 15 min | Envision™ | Ready-to-Use Kit | Dako, Glostrup, Denmark |
p16Ink | Monoclonal, clone G175-405 | 98°C Hot water, pH 6.1, | 20 min | Envision™ | 1:10 | BD Biosciences, Heidelberg, Germany |
Ki67 | Monclonal, clone MIB-1 | 98°C Hot water, pH 9.0, | 15 min | Envision™ | Ready-to-Use Kit | Dako, Glostrup, Denmark |
BRAF | Monoclonal, clone VE1 | 37°C Hot water, PH 9.0 | 30 min | Envision™ | 1:100 | DCS Innovative Diagnostik, Hamburg, Germany |
MLH1 | Monoclonal, clone ES05 | 98°C, pH 9.0 | 20 min | Envision™ | Ready-to-Use Kit | Dako, Glostrup, Denmark |
Beta-catenin | Monoclonal | 37°C water bath 30 min | 20 min | Zytochem Plus HRP Polymer (Zytomed Systems, Berlin, Germany) | 1:2000 | BD Biosciences, Heidelberg, Germany |
CK20 | Monoclonal, Clone Ks20.8 | 98°C, pH 9.0 | 30 min | Envision™ | Ready-to-Use Kit | Dako, Glostrup, Denmark |