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Table 1 Digital image analysis outputs and pathologist’s IHC HER2 score on the IHC multi-block tissue controls

From: Digital immunohistochemistry: new horizons and practical solutions in breast cancer pathology

 

Core #1 HER2 0

Core #2 HER2 1+

Core #3 HER2 2+

Core #4 HER2 3+

Cores #1-4 Mean

Mean membrane intensity*

176 ± 16

190 ± 7

155 ± 11

86 ± 11

152 ± 8

% of cells with complete membranous staining*

1 ± 4

2 ± 5

17 ± 6

59 ± 6

19 ± 4

Total number of cells*

4277 ± 3205

6759 ± 3342

17536 ± 3382

19803 ± 4269

12094 ± 2778

Pathologist’s HER2 staining intensity score corresponding the expected category**

87/88

73/89

88/91

84/87

N/A

  1. * Mean ± standard deviation
  2. ** Data represented as a ratio of number of corresponding HER2 score/all cores evaluated. In all discordant cases, except in the category of Core#1 HER2 0, pathologist gave a lower intensity score than that expected for the category. Total number of IHC control slides evaluated was 91, except some tissue cores were considered inadequate by the pathologist (number of cores represented in the denominator).
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Diagnostic Pathology

ISSN: 1746-1596

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