Figure 2

2-Color RNA Detection Methodology. A cocktail of antisense hapten-labeled riboprobes, targeting the constant region of either KAPPA or LAMBDA mRNA, was hybridized to de-paraffinized protease treated tissue samples. Following stringency washes the haptens were sequentially detected using a cognate anti-hapten monoclonal antibody conjugated to HRP which catalyzed deposition of a tyramide-hapten conjugate. Following inactivation of the HRP conjugate, each amplified hapten was then sequentially detected using a second anti-hapten antibody-HRP conjugate which catalyzed silver or pink chromogen deposition.