Figure 2

Photomicrographs of patient 1 (A-F), patient 2 (G), and patient 4 (H). A. Nodules of adrenal cortical tumor cells separated by an infiltrate of paler spindle cells in patient 1 (400x). B. Adrenal cortical tumor cells showing numerous brightly eosinophilic inclusions of varying sizes in the cytoplasm. Multiple inclusions are present in each cell (400x). C. Spindle cell infiltrate composed of degenerating adrenal cortical cells (400x). D. Inclusions were also present in the zona fasciculata directly adjacent to the tumor (400x). E. Inclusions positive for PAS, showing bright pink staining, with Luxol Fast Blue counterstaining. There were variably sized multiple intracellular inclusions (400x). F. Hemoglobin stain with a spectrum of staining including olive green, pale pink, and brick red inclusions. Multiple inclusions of each color were present in a single cell (400x). G. In patient 2, large eosinophilic bodies with concentric rings were found single per cell throughout the tumor (400x). H. In patient 4, rare large, eosinophilic, concentrically laminated bodies were seen within the zona glomerulosa (400x).