Figure 1From: Whole DNA methylome profiling in lung cancer cells before and after epithelial-to-mesenchymal transitionCharacteristics of the A549 cell line before and after EMT. (a) The morphology of A549 cells treated with TGF-β (5 ng/ml) for different time intervals was visualized by phase-contrast microscopy (Olympus). (b) The expression of E-cadherin, N-cadherin, Vimentin, and Snail in TGF-β-treated A549 cells was analyzed by western blotting. (c) mRNA extracted from A549 cells treated with TGF-β was analyzed by RT-PCR (E-cadherin is shown on the left y-axis, and N-cadherin, Vimentin, and Snail1 are shown on the right y-axis).Back to article page