Table 1 Characteristics of the antibodies utilised in the study
Antibody name | Clone | Company | Dilution | Staining pattern |
|---|---|---|---|---|
MMP1 | 3B6 | Santa Cruz Biotechnology, Inc. Dallas, USA | 1:25 | Nuclear |
MMP2 | 2C1 | Santa Cruz Biotechnology | 1:25 | Nuclear |
MMP3 | 1B4 | Santa Cruz Biotechnology | 1:25 | Nuclear, cytoplasmic, amyloid |
MMP9 | 2C3 | Santa Cruz Biotechnology | 1:25 | Nuclear, cytoplasmic, amyloid |
E-cadherin | NCH-38 | Dako, Glostrup Denmark | 1:30 | Membranous, cytoplasmic |
N-cadherin | 6G11 | Dako | 1:30 | Membranous, nuclear |
P- cadherin | H-105 | Santa Cruz Biotechnology | 1:30 | Membranous, cytoplasmic, nuclear |
Integrin β1 | 4B7R | Santa Cruz Biotechnology | 1:30 | Membranous (lost in some areas), cytoplasmic |
Beta-catenin | β-catenin-1 | Dako | 1:100 | Membranous, cytoplasmic |
Vimentin | SP20 | Thermo Fisher Scientific, Waltham, USA | 1:200 | Cytoplasmic |
Twist 1 | 10E4E6 | Leica Biosystems, Wetzlar, Germany | 1:1000 | Nuclear |
Slug 1 | 1A6 | Novus Biologicals, Abingdon, UK | 1:50 | Nuclear, cytoplasmic |
CXCR4 | polyclonal | Thermo Scientific | 1:500 | Membranous, cytoplasmic, nuclear |
Podoplanin | D2-40 | Dako | 1:50 | Membranous |
Collagen IV | CIV 22 | Dako | 1:50 | Basement membranes |
Ki-67 | MIB-1 | Dako | 1:50 | Nuclear |
Cytokeratin 5/6 | D5/16 B4 | Dako | 1:50 | Cytoplasmic |
Cytokeratin19 | b170 | Leica Biosystems | 1:150 | Cytoplasmic |
P63 | 7JUL | Leica Biosystems | 1:25 | Nuclear |
S100 | polyclonal | Dako | 1:500 | Cytoplasmic, nuclear |
Actin (Smooth Muscle | 1A4 | Dako | 1:40 | Cytoplasmic |
Actin, Smooth Muscle | polyclonal | Thermo Scientific | 1:100 | Cytoplasmic |