Fig. 4

Inhibition of miR-1322 reversed the effects of circ_0001715 knockdown on NSCLC cell proliferation and apoptosis in A549 and H1299 cells. The expression of miR-1322 was assessed by RT-qPCR after transfection of anti-miR-1322 and anti-miR-NC (A). The proliferation ability of A549 and H1299 cells that transfected with si-NC, si-circ_0001715, si-circ_0001715 + anti-NC, si-circ_0001715 + anti-miR-1322 was detected by the clone formation assay, EDU assay and wound healing assay (B-D). Sphere formation assay was applied for rescue experiments in A549 and H1299 cells to assess whether the biological function of circ_0001715 in cells could be affected by miR-1322 after transfected with si-NC, si-circ_0001715, si-circ_0001715 + anti-NC, si-circ_0001715 + anti-miR-1322 (E). Apoptosis rate of A549 and H1299 cells were presented by flow cytometry assay transfected with si-NC, si-circ_0001715, si-circ_0001715 + anti-NC, si-circ_0001715 + anti-miR-1322 (F). Western blotting assay showed that circ_0001715 silencing on PCNA and Bax in A549 and H1299 cells was reversed by miR-1322 inhibitor transfected with si-NC, si-circ_0001715, si-circ_0001715 + anti-NC, si-circ_0001715 + anti-miR-1322 (G and H). Effect of the above cell lines on the tube formation ability of HUVECs was determined by tube formation assay (I). **p < 0.01, ***p < 0.001