Fig. 5

CANT1 was a target gene of miR-1322 and circ_0001715 could regulate the expression of CANT1 through miR-1322. The putative binding sites of miR-1322 on CANT1 were shown (A). The expression of CANT1 was measured by RT-qPCR and western blotting assay (B). The correlation between CANT1 and miR-1322 or circ_0001715 in LUAD tissues was determined by Pearson’s correlation analysis (C and D). CANT1 protein levels in LUAD tissues and cell lines were detected by RT-qPCR (E and F). Dual-luciferase reporter assay was used to demonstrate the relationship between miR-1322 and CANT1 (G and H). The expression of CANT1 was assessed by western blotting assay after transfection of anti-miR-1322 (I and J). The effect of circ_0001715 silencing and miR-1322 inhibitor on the protein level of CANT1 in A549 and H1299 cells was illustrated by western blot assay (K and L). **p < 0.01, ***p < 0.001