Fig. 5
From: Completing the view – histologic insights from circular AAA specimen including 3D imaging
Patient 4 sample acquisition, rupture site and histomorphology. CT-angiogram demonstrates the approx. site of sample acquisition. The arrow marks the aneurysm sac incision during open repair. Photo shows the intraoperative situs with the rupture site (asterisk) at the dorsal right part of the aneurysm sac (iliac arteries marked with red vessel loops. The histologic photos show a complete circular preparation of the aneurysm sac (HE) oriented in line with the CT-angiogram. Cutouts are presented from the right lateral wall (upper panel) and the rupture site (lower panel). EvG stainig demonstrates fragmented elastic fibers and matrix remodeling as described above for the lateral wall and an amorphous bleb-like structure with massive intramural hemorrhage (#) at the rupture site. At the outer margins of this structure, CD45 and CD20 positive cells are found in the adventitia. For all histologic photos the aortic lumen is oriented upwards. All samples are presented in the same orientation (A = anterior, P = posterior, R = right, L = left, H = head, T = tail) (scale bar 5 mm for HE; cutouts upper panel 250 µm; cutouts lower panel 500 µm)