Early uptake and continuous accumulation of thallium-201 chloride in a benign mixed tumor of soft tissue: Case Report
© Nagata et al; licensee BioMed Central Ltd. 2010
Received: 25 February 2010
Accepted: 30 May 2010
Published: 30 May 2010
A case of benign mixed tumor of the soft tissue in a 64-year-old Japanese male is presented. He noticed a painless, elastic hard mass sized 3 cm in the right knee, which gradually grew larger and harder in the last 5 years. Magnetic resonance imaging demonstrated a mass lesion embedded in the subcutaneous tissue with low and high signal intensity at T1- and T2-weighted images, respectively. Tl-201 scintigraphy showed an early uptake of Tl-201 within the lesion at 10 minutes after injection, which was slightly decreased but still continued at 2 hours later. The patient underwent a resection of tumor, and the pathological diagnosis was a benign mixed tumor of soft tissue without high vascularity, characterized by histological features similar to pleomorphic adenomas in the salivary glands. Immunohistochemical study proved expression of Na+/K+-ATPase of tumor cells. Overexpression of Na+/K+-ATPase of the tumor might be responsible for the early uptake of Tl-201, and poor vascular structure in this tumor might lead to continuous accumulation. The Tl-201 scintigraphic features of mixed tumor of soft tissue are assessed to resemble those of malignant soft tissue tumors.
Mixed tumors can occur in soft tissue as well as in the skin and soft palate [1, 2], although only 18 cases have been described to be primary in soft tissue [3, 4]. Mixed tumors of soft tissue demonstrate similar histological features to pleomorphic adenomas of the salivary glands, having both benign and malignant forms in the same way. The feature of thallium-201 chloride (Tl-201) scintigraphy in mixed tumors of soft tissue is still unknown. Herein, we present a case of benign mixed tumor arising in the subcutis, which demonstrated an early uptake of Tl-201 with slightly decreased accumulation in the delayed scan. Immunohistochemical study proved the tumor cells to express sodium/potassium (Na+/K+)-ATPase, which is considered to play an important role in uptake and accumulation of Tl-201 [5–8].
Comprehensive immunohistochemical findings are demonstrated in Figure 3. The epithelial component of the tumor was diffusely stained with cytokeratin (CK) AE1/AE3 and CAM5.2, and partially with CK 34βE12. Epithelial membrane antigen (EMA) was strongly expressed on the luminal surface of glandular structures. Mesenchymal tumor cells were totally negative for these epithelial markers except scattered cells positive for CAM5.2. Desmin and α-smooth muscle actin were not expressed within the tumor. Glandular component was positive for anti-p63, indicating the basal myoepithelial characteristics of the cells. Many mesenchymal tumor cells were stained with anti-S-100 protein. Vascular structures indicated by CD31 and CD34 were scant in the stroma. Bcl2 was strongly expressed in the epithelial component with a weaker intensity in the mesenchymal component. Epithelial tumor cells were faintly stained with anti-p16 in the cytoplasm. Ki-67 labeling index was less than 1% and p53-positive cells were rarely seen in both epithelial and mesenchymal lesion, indicating the low proliferation rate and the absence of disruption of p53 tumor-suppressor pathway. These findings indicated that the tumor to be composed of epithelial and mesenchymal lesions, and the epithelial component having myoepithelial characteristics. The pathological diagnosis was a benign mixed tumor arising in the subcutis of the knee. All the primary antibodies used for the differential diagnosis were from Dako A/S (Glostrup, Denmark) except CAM5.2 (Becton Dickinson, Flanklin Lakes, NJ, USA) and anti-p16 (Novocastra Laboratories Ltd., Newcastle upon Tyne, UK).
Well-characterized as "pleomorphic adenomas" in salivary glands, mixed tumors may also occur in the skin and soft palate, and have been recently recognized to be primary in soft tissue [1, 2]. Microscopically, mixed tumors of soft tissue show similar morphologic feature to their salivary gland counterparts. Mixed tumors, myoepitheliomas, and parachordomas originated from the soft tissue share many clinical and histological features, all of which are considered within the same spectrum. The vast majority of these tumors arise in the subcutaneous or deep subfascial soft tissue of extremities, more frequently in the upper than the lower , usually affect adults with a significant number of children [3, 9]. Only 18 cases of mixed tumors of soft tissue were reported , and 33 percent of those were described to meet histological criteria for malignancy resulting in local recurrence or metastasis .
In the present case, diagnostic differential considerations include other benign lesions, such as neurilemoma of degenerative type and organized hematoma that may radiologically demonstrate a granular pattern of low in high signal intensity on T2-weighted MRI images indicating calcification or hemorrhage within the tumor. However, these diagnoses were excluded by the histological finding of tumor cells showing both epithelial and mesenchymal characteristics. Given the morphologic heterogeneity of this tumor, the differential diagnosis is quite broad and depends in large part upon the predominant cell type and stromal component [10, 11]. Other candidate was synovial sarcoma with both epithelial and mesenchymal lesion in the tumor expressing bcl-2. Although the present case showed strong bcl2 expression in the cytoplasm, however diagnosis of synovial sarcoma was deniable as the epithelial cells showed myoepithelial marker p63. Metastasis of myoepithelial carcinoma originated from other organs, such as head and neck might be excluded by the benign histology of the tumor.
This is the first report of Tl-201 scintigraphic features in a benign mixed tumor of soft tissue in our reviewing literatures. It is generally considered that Tl-201 uptake around tumor cells mainly depends on the volume of blood flow and blood pools . In the second step, Tl-201 has similar ion-radius and physical effects similar to potassium , having five times the affinity to cell as potassium, and is transported into the cell instead of potassium as a result . The preferential transportation of Tl-201 mainly depends on Na+/K+-ATPase, which is overexpressed in a variety of malignant tumors [5–8] and some benign tumor cells . In the present case, although vascularity of tumor was not high by morphological assessment, imunohistochemical study revealed expression of Na+/K+-ATPase in most tumor cells. This result may account for a mechanism of the early uptake and continuous accumulation of Tl-201 in a benign mixed tumor of soft tissue.
Conclusive significance of thallium-201 scintigraphy in soft tissue tumors has been still controversial within our knowledge. Positive examination often indicates malignant tumors, but some benign tumors are occasionally estimated as positive [15, 16]. Accumulation of Tl-201 at delayed scan has been reported as a more sensitive finding distinguishing malignancies from benignancies . In our case, we considered the continuous accumulation of Tl-201 at delayed scan as a potential sign indicating malignancy, although it was denied by histological examination including immunohistochemistry.
We accessed Tl-201 scintigraphic features of a mixed tumor of soft tissue showing an early uptake and longtime accumulation of Tl-201. Overexpression of Na+/K+-ATPase in the tumor was considered to be responsible for the early uptake followed by continuous accumulation of Tl-201 due to poor vascular structure of the tumor. We emphasize that clinicians should include such benign tumor when they find ambiguous Tl-201 scintigraphic feature.
Written informed consent was obtained from the patient for publication of this case report and any accompanying images. A copy of the written consent is available for review by the Editor-in-Chief of this journal.
The authors thank Yoshimi Yamaguchi, Junichi Ashimura, Kikuichi Nakagawa, Reiko Kitazume (Department of Pathology, Osaka Medical Center for Cancer and Cardiovascular Diseases) for technical assistance.
- Kilpatrick SE, Hitchcock MG, Kraus MD, Calonje E, Fletcher CD: Mixed tumors and myoepitheliomas of soft tissue: a clinicopathologic study of 19 cases with a unifying concept. Am J Surg Pathol. 1997, 21: 13-22. 10.1097/00000478-199701000-00002.View ArticlePubMedGoogle Scholar
- Michal M, Miettinen M: Myoepitheliomas of the skin and soft tissues. Report of 12 cases. Virchows Arch. 1999, 434: 393-400. 10.1007/s004280050358.View ArticlePubMedGoogle Scholar
- Hornick JL, Fletcher CD: Myoepithelial tumors of soft tissue: a clinicopathologic and immunohistochemical study of 101 cases with evaluation of prognostic parameters. Am J Surg Pathol. 2003, 27: 1183-1196. 10.1097/00000478-200309000-00001.View ArticlePubMedGoogle Scholar
- Lee JR, Georgi DE, Wang BY: Malignant myoepithelial tumor of soft tissue: a report of two cases of the lower extremity and a review of the literature. Ann Diagn Pathol. 2007, 11: 190-198. 10.1016/j.anndiagpath.2006.04.004.View ArticlePubMedGoogle Scholar
- Delpire E, Gullans SR: Cell volume and K+ transport during differentiation of mouse erythroleukemia cells. Am J Physiol. 1994, 266: C515-523.PubMedGoogle Scholar
- Diomede L, Bianchi R, Modest EJ, Piovani B, Bubba F, Salmona M: Modulation of ATPase activity by cholesterol and synthetic ether lipids in leukemic cells. Biochem Pharmacol. 1992, 43: 803-807. 10.1016/0006-2952(92)90246-F.View ArticlePubMedGoogle Scholar
- Numazawa S, Shinoki MA, Ito H, Yoshida T, Kuroiwa Y: Involvement of Na+, K(+)-ATPase inhibition in K562 cell differentiation induced by bufalin. J Cell Physiol. 1994, 160: 113-120. 10.1002/jcp.1041600114.View ArticlePubMedGoogle Scholar
- Brismar T, Collins VP: Potassium and sodium channels in human malignant glioma cells. Brain Res. 1989, 480: 259-267. 10.1016/0006-8993(89)90191-1.View ArticlePubMedGoogle Scholar
- Kilpatrick ES, Limon J: Mixed tumour/myoepithelioma/parachordomas. Pathology and Genetics of Tumours of Soft Tissue and Bone, World Health Organization Classification of Tumours. Edited by: Fletcher CD, Unni KK, Mertens F. 2002, Lyon, France: IARCpress, 198-199.Google Scholar
- Armah HB, McGough RL, Goodman MA, Gollin SM, Surti U, Parwani AV, Rao UN: Chondromyxoid fibroma of rib with a novel chromosomal translocation: a report of four additional cases at unusual sites. Diagn Pathol. 2007, 2: 44-10.1186/1746-1596-2-44.PubMed CentralView ArticlePubMedGoogle Scholar
- Martorell M, Pérez-Vallés A, Gozalbo F, Garcia-Garcia JA, Gutierrez J, Gaona J: Solitary fibrous tumor of the thigh with epithelioid features: a case report. Diagn Pathol. 2007, 2: 19-10.1186/1746-1596-2-19.PubMed CentralView ArticlePubMedGoogle Scholar
- Sato T, Indo H, Kawabata Y, Iwashita Y, Morita Y, Noikura T, Sugihara K: Dynamic scintigraphy with thallium-201 chloride (Tl-201) for the diagnosis of tumors of the head and neck. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2001, 92: 228-235. 10.1067/moe.2001.116507.View ArticlePubMedGoogle Scholar
- Takekawa H, Itoh K, Abe S, Ogura S, Isobe H, Furudate M, Kawakami Y: Thallium-201 uptake, histopathological differentiation and Na-K ATPase in lung adenocarcinoma. J Nucl Med. 1996, 37: 955-958.PubMedGoogle Scholar
- Sato T, Indo H, Kawabata Y, Kobayashi T, Suenaga S, Iwashita Y, Nitta T, Sugihara K, Majima HJ: Thallium-201 chloride (Tl-201) accumulation and Na+/K+-ATPase expression in tumours of the head and neck. Dentomaxillofac Radiol. 2005, 34: 212-217. 10.1259/dmfr/50773431.View ArticlePubMedGoogle Scholar
- Sato O, Kawai A, Ozaki T, Kunisada T, Danura T, Inoue H: Value of thallium-201 scintigraphy in bone and soft tissue tumors. J Orthop Sci. 1998, 3: 297-303. 10.1007/s007760050056.View ArticlePubMedGoogle Scholar
- Sugawara Y, Kikuchi T, Kajihara M, Semba T, Ochi T, Fujii T, Mochizuki T, Sakayama K, Nakata S: Thallium-201 scintigraphy in bone and soft-tissue tumors: a comparison of dynamic, early and delayed scans. Ann Nucl Med. 2005, 19: 461-468. 10.1007/BF02985573.View ArticlePubMedGoogle Scholar
- Goto Y, Ihara K, Kawauchi S, Ohi R, Sasaki K, Kawai S: Clinical significance of thallium-201 scintigraphy in bone and soft tissue tumors. J Orthop Sci. 2002, 7: 304-312. 10.1007/s007760200052.View ArticlePubMedGoogle Scholar
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