The wound healing process is a normal physiological response to injury and generally leads to restoration of normal structure and function in damaged tissues. In certain disorders, the wound healing process leads to an altered restitution of tissue structure and function that is associated with the development of remodeling and fibrosis [1, 2].
Kombucha is a traditional beverage consumed in various parts of the world and especially in Asia. Its earliest known use originated with Dr. Kombu in 220 BC for curing the digestive troubles of Japan’s emperor. Many health promoting effects of Kombucha have been claimed , but adverse effects attributed to this beverage have been reported as well . Kombucha is typically prepared by fermenting black tea, sweetened with sugar, with what is popularly known as a ‘tea fungus,’ at room temperature for 10–12 days [5, 6]. This so called ‘tea fungus’ is actually a symbiosis of yeasts and acetic acid bacteria, the cellulosic pellicle formed by the latter commonly described as the ‘fungus’ [7–9].
Currently, Kombucha is alternately praised as “the ultimate health drink” [9, 10] or damned as “unsafe medicinal tea” [10–12]. Testimonials claim causal benefits for everything from AIDS improvement, balding relief, cancer cures, and diabetic and arthritic symptom relief to prolonged longevity, weight loss, and cognition enhancements [13, 14]. In conjunction with Kombucha’s growing popularity, life-threatening human health problems are beginning to be reported. Hepatotoxicity,  necrotizing pancreatitis, 16 and several human deaths have been linked to drinking Kombucha beverage [11, 16, 17].
In traditional medicine Kombucha was used for many disease treatments, including wound healing. Because of high abilities of Kombucha in curing, antibiotic, detoxification and skin luminosity it was supposed to determine the effect on injured skin in vitro condition. The composition and properties of tea are well documented, but scarce scientific information is available concerning the composition and the effects of Kombucha on health. Benefits have been reported by testimony of users in different conditions and with variable consummation. Nitrofurazone, an antibacterial drug was found to be photolabile; its photolability has been described in a number of recent papers [17, 18].
Nitrofurazone is a synthetic nitrofuran with abroad antibacterial spectrum; it acts by inhibiting bacterialenzymes involved in carbohydrate metabolism . Nitrofurazone is a topical anti-infective agent with a broadantibacterial spectrum; bactericidal against most bacteria commonly causing surface infections, including S aureus, Streptococcus.
Wound healing is a dynamic process that involves the integrated action of a number of cell types (the interactions of many cells types, including inflammatory cells, fibroblasts, keratinocytes and endothelial cells, as well as the involvement of growth factors and enzymes), the extra cellular matrix, and soluble mediators termed cytokines. The normal adult healing process proceeds through a number of overlapping events: clot formation, inflammation, re-epithelialization, angiogenesis, granulation tissue formation, wound contraction, scar formation and tissue remodeling. Growth factors released in the traumatized area promote cell migration into the wound area (chemotaxis), stimulate the growth of epithelial cells, and fibroblasts (mitogenesis) initiate the formation of new blood vessels (angiogenesis) and stimulate matrix formation and remodeling of the affected region. The formation of new blood vessels from pre-existing capillaries that are then able to penetrate the wound site is an essential component of the wound healing process [21–23].
Until now, no knowledge has been available pertaining to the histological study of Kombucha tea treatment on wound healing in injured skin. The aim of the present study was to use histopathological and clinical methods to evaluate the effect of Kombucha tea and Nitrofurazone on cutaneous full-thickness wounds healing in rats.