An unusual combined thymic carcinoma composed of squamous cell carcinoma and type AB thymoma: a rare case report
© The Author(s). 2017
Received: 6 June 2016
Accepted: 7 December 2016
Published: 17 January 2017
Combined thymic carcinoma is a malignant neoplasm of the thymus recently added to the 4th edition of the World Health Organization (WHO) classification of tumors of the lung, pleura, thymus and heart. It involves at least one type of thymic carcinoma and another thymic epithelial tumor. The previously used term “combined thymic epithelial tumor” has been abandoned.
Here, we present an unusual case of combined thymic carcinoma of the thymus in a 44-year-old male who had suffered from fever, chest pain, chest tightness and shortness of breath. Magnetic resonance imaging (MRI) detected a mass approximately 6.4 cm × 4.2 cm in the anterior mediastinum, and a nonencapsulated tumor approximately 5.0 cm × 3.5 cm × 2.5 cm with an irregular shape was resected. The morphological features and the immunostaining pattern of the tumor revealed it to be an unusual combined thymic carcinoma consisting of type AB thymoma and squamous cell carcinoma. There were cysts of various sizes, some of which had crack-like structures, in the type AB thymoma area. A gradual transition could be seen between these structures and the squamous cell carcinoma, indicating that the carcinoma portion may have originated from the composition of the thymoma.
Combined thymic carcinoma composed of type AB thymoma and squamous cell carcinoma is rare, and the carcinoma portion may have originated from epithelial structures in the type AB thymoma.
KeywordsCombined thymic carcinoma Mediastinum Squamous cell carcinoma Type AB thymoma
Combined thymic carcinomas are tumors composed of at least one type of thymic carcinoma and another thymic epithelial tumor of any type of thymoma or thymic carcinoma, excluding small cell carcinoma and large cell neuroendocrine carcinoma . It was proposed that the previously recommended term “combined thymoma” be abandoned because thymomas of different histological types within the same tumor are very common, according to the 4th edition of the World Health Organization (WHO) classification of tumors of the lung, pleura, thymus and heart . Instead, the WHO suggested that all the histological types present be listed in the diagnosis . For heterogeneous tumors that consist of a thymic carcinoma and a thymoma, the organization proposes the new term “combined thymic carcinoma.” The most common combinations are thymic squamous cell carcinoma and type B3 thymoma and papillary adenocarcinoma or sarcomatoid carcinoma associated with type A thymoma [1–4]. The different components of the tumors may show a gradual transition or be sharply separated from each other . Thymic carcinoma occasionally arises in type A thymoma, but combined thymic carcinoma composed of squamous cell carcinoma and type AB thymoma is extremely rare. Here, we present a case of combined thymic carcinoma composed of type AB thymoma and squamous cell carcinoma using the diagnostic criteria of the 4th edition of the WHO classification of tumors of the lung, pleura, thymus and heart.
A 44-year-old man was referred to our hospital with a 1-month history of fever without an obvious cause. In that period, after he had taken anti-inflammatory agents the fever subsided to some extent. However, in the week before presenting to the hospital for diagnosis and treatment, he experienced chest pain, chest tightness, shortness of breath, severe cough and blood in phlegm accompanied by vomiting after tiredness. In examination at the hospital, he reported no muscle weakness. Blood tests showed neuron-specific enolase (NSE) (19.71 ng/ml), CA125 (49.61 μ/ml) and Cyfra21-1 (8.42 ng/ml) levels slightly higher than normal, whereas his CEA and CA199 levels were normal. The patient underwent surgery, and the tumor was found to have invaded the local pericardium. No lymph node metastasis or distant metastasis was detected. The patient showed no recurrence 6 months after the surgery.
Materials and methods
The resected samples of the tumor were embedded in paraffin blocks. The blocks were cut into sections and stained with alum hematoxylin and eosin for morphological examination under light microscope. The immunohistochemistry was examined using an SP-kit (Maixin Biotechnology, Fuzhou, Fujian, China) according to the instruction manual of the developer. The sections were incubated overnight at 4 °C with the following primary antibodies: CD1α (1:100, DAKO), CD3 (1:100, DAKO), CD5 (1:200, DAKO), CD20 (1:100, DAKO), CD99 (1:200, DAKO), CK (Pan) (1:200, DAKO), CK19 (1:200, DAKO), CK20 (1:100, DAKO), Ki-67 (1:200, DAKO), p63 (1:100, DAKO) and TdT (1:100, DAKO). This study was prospectively performed and approved by the institutional ethics committees of China Medical University and conducted in accordance with the ethical guidelines of the Declaration of Helsinki.
Imaging and gross features
Primary thymic carcinomas are rare malignant neoplasms of the mediastinum, and combined thymic carcinomas are even less common [1–4]. Almost all reported cases of combined thymic carcinomas were located in the anterior mediastinum . The clinical features of the tumors are the same as those of thymic epithelial tumors of a single histological type [1, 5, 6]. Most components of carcinoma in combined thymic carcinomas are squamous cell carcinomas, and type B2 and B3 thymomas account for most of these components [1–4]. Thymic carcinoma combined with type A thymoma is rare, but combined thymic carcinoma composed of squamous cell carcinoma and type AB thymoma is even less common. The macroscopic features of combined thymic carcinoma are the same as those of tumors of a single histological type [1–4]. The case reported here was diagnosed as an unusual combined thymic carcinoma composed of type AB thymoma and squamous cell carcinoma according to the 4th edition of the WHO classification of tumors of the lung, pleura, thymus and heart. This type of thymic tumor is new to this edition, and it is suggested that the formerly used term “combined thymic epithelial tumor” not be used anymore because thymomas showing different histological types in the same tumor are very common.
In the current case, histological and immunohistochemical findings revealed a combination of type AB thymoma and squamous cell carcinoma. There were cysts of various sizes, some of which had crack-like structures in the type AB area of thymoma, with a gradual transition between these structures and the squamous cell carcinoma. This indicates that the squamous cell carcinoma may have originated from the composition of the thymoma tissue. However, whether the carcinoma components originated from the epithelial cells in the thymomas and what mechanisms are involved remain unclear. Here, we found that CD5 and CD117 were negative in thymic carcinoma. Metastatic carcinoma needed to be ruled out. According to the 4th edition of the WHO classification of tumors of the lung, pleura, thymus and heart, the positive rates of CD5 and CD117 in thymic squamous cell carcinoma are 74% and 84%, respectively . The physical examination of the patient did not indicate the presence of tumor in other part of the body other than the anterior mediastinum. The pathological findings indicated a transition between the squamous cell carcinoma and the cysts in the type AB thymoma. These findings suggest that the squamous cell carcinoma was originally part of a combined thymic tumor rather than a metastatic tumor. Thymic carcinoma can arise from thymic cysts. In this case, we found numerous structures of cysts in the portion of the type AB thymoma in the tumor tissue. There was a transition between this section and the squamous cell carcinoma. It indicated that there was a close relationship between these different tumor parts and that the portion of carcinoma may have originated in the thymoma. However, it does not entirely exclude the possibility that the carcinoma may have originated from a thymic cyst because the cyst might also have come from the structures of the cysts in the thymoma. Actually, in this case, the squamous cell carcinoma showed cystic change and hemorrhage, although we did not find a clear lesion involving a thymic cyst. Interestingly, CD5 and CD117 were both negative in the squamous cell carcinoma, which is often positive for these two markers. Whether there are different features of these two markers in the squamous cell carcinoma that originated from a thymic cyst compared with that in thymic squamous carcinoma needs to be further clarified. Moreover, the origination and oncogenesis of thymic squamous carcinoma are not fully understood. No systematic study has been done on the genetic changes in the components of combined thymic carcinoma to provide clues and evidence to answer these questions. Some studies have shown that the genetic alterations found in thymic squamous carcinomas of the thymus are distinctive from those in tumors of the lung, head, and neck, although their morphological features are similar [7–9].
Combined thymic carcinomas are epithelial thymic tumors that consist of at least one type of thymic carcinoma and another thymic epithelial tumor. Although most of these tumors are composed of squamous cell carcinoma with type B2 or B3 thymomas, our study indicates that type AB thymoma can also be combined with squamous cell carcinoma in the anterior mediastinum. The gradual transition of the cystic structures in type AB thymoma with squamous cell carcinoma points to the possible origin of the malignant part of the thymoma, although systematic genetic analysis is needed to understand the etiology and carcinogenesis of these tumors.
Magnetic resonance imaging
World Health Organization
This work was supported by the National Natural Science Foundation of China (no. 81472599 to Chuifeng Fan, MD).
Availability of data and materials
All data generated or analyzed during this study are included in this published article.
YF J, Y L, and CF F participated in the histopathological evaluation, performed the literature review, acquired the photomicrographs and drafted the manuscript. YF J, XY S and CF F performed the immunohistochemical stain evaluation. YF J, Y Z and XY M conceptualized and designed the study. YF J, Y L and CF F provided the final histopathological diagnoses, and CF F revised the manuscript. All the authors read and approved the final manuscript.
The authors declare that they have no competing interests.
Consent for publication
Written informed consent was obtained from the patient for publication of this case report and the accompanying images.
Ethics approval and consent to participate
The institutional ethics committees of China Medical University approved the study.
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- Travis WD, Brambilla E, Burke AP, Marx A, Nicholson AG. WHO classification of tumors of the lung, pleura, thymus and heart. 4th ed. Lyon: IRAC; 2015. p. 242.Google Scholar
- Suster S, Moran CA. Primary thymic epithelial neoplasms showing combined features of thymoma and thymic carcinoma. A clinicopathologic study of 22 cases. Am J Surg Pathol. 1996;20(12):1469–80.View ArticlePubMedGoogle Scholar
- Matsuno Y, Rosai J, Shimosato Y. Papillary adenocarcinoma. WHO classification of tumors. Pathology and genetics of tumors of the lung, pleura, thymus and heart. 3rd ed. Lyon: IRAC; 2004. p. 183.Google Scholar
- Weissferdt A, Moran CA. Thymic carcinoma, part 1: a clinicopathologic and immunohistochemical study of 65 cases. Am J Clin Pathol. 2012;138(1):103–14.View ArticlePubMedGoogle Scholar
- Okamoto T, Miyazaki Y, Sakakibara Y, Tamaoka M, Sumi Y, Inase N. Successful diagnosis of a combined thymic epithelial tumor by endobronchial ultrasound-guided transbronchial needle aspiration. J Med Dent Sci. 2011;58(4):123–6.PubMedGoogle Scholar
- Weissferdt A, Moran CA. Thymic carcinoma, part 2: a clinicopathologic correlation of 33 cases with a proposed staging system. Am J Clin Pathol. 2012;138(1):115–21.View ArticlePubMedGoogle Scholar
- Travis WD, Brambilla E, Müller-Hermelink HK, Haris CC. World Health Organization Classification of Tumours. Pathology and Genetics of Tumours of the Lung, Thymus and Heart. Lyon: IARC Press; 2004. p. 247.Google Scholar
- Girard N, Shen R, Guo T, et al. Comprehensive genomic analysis reveals clinically relevant molecular distinctions between thymic carcinomas and thymomas. Clin Cancer Res. 2009;15:6790–9.View ArticlePubMedPubMed CentralGoogle Scholar
- Ströbel P, Hohenberger P, Marx A. Thymoma and thymic carcinoma: molecular pathology and targeted therapy. J Thorac Oncol. 2010;5(10 Suppl 4):S286–90.View ArticlePubMedGoogle Scholar