Differential diagnosis of laryngeal spindle cell carcinoma and inflammatory myofibroblastic tumor – report of two cases with similar morphology

Table 1 Immunohistochemistry

Antibody	Expression in SPC	Expression in IMT	Source/Dilution
First stainings
Vimentin *	++	++	DAKO, mouse, 1:800
PanCytokeratin AE1/3 *	+	NR	DAKO, mouse, 1:100
EMA	+	+ (weak only)	LOXO, mouse, 1:20
ALK-1 *	NR	+	DAKO, mouse, 1:20
smooth muscle Actin	++	NR	Beckman Coulter, mouse, 1:20
Desmin	NR	NR	DAKO, mouse, 1:400
S100	NR	NR	DAKO, rabbit, 1:2000
CD34	NR	NR	DAKO, mouse, 1:100
CD117	NR	NR	DAKO, mouse, 1:100
Ki67 *	++ 60–80%	+ 5–10%	DAKO, mouse, 1:200
Additional stainings
PanCytokeratin KL1 *	+	NR	Beckman Coulter, mouse, 1:40
PanCytokeratin MNF116	NR (but epithelium +)	NR	DAKO, mouse, 1:50
Cytokeratin 5/6	+ (weak only)	NR	DAKO, mouse, 1:50
Cytokeratin 7	NR	NR	DAKO, mouse, 1:20
CD68	NR	NR	Kiel, ascites, 1:20000
CD30	NR	NR	DAKO, mouse, 1:5
CD56	NR	NR	DAKO, mouse, 1:10
Her2Neu	NR	NR	DAKO, rabbit, 1:100
Estrogen receptor	NR	NR	DAKO, mouse, 1:10
Progesteron receptor	NR	NR	DAKO, mouse, 1:10
p53	+	+	DAKO, mouse, 1:20
p63 *	++	NR	Neomarkers, mouse, 1:200
p21	++	++	Dianova, rat, 1:20
Cyclin D1	+	+	LOXO, mouse, 1:20
Bcl-2	+ (weak cytoplasmatic)	++	DAKO, mouse, 1:400
Rb	+	++	Neomarkers, mouse 1:50
HHV 8	NR	NR	Tebu, rat, 1:200
HPV	NR	NR	Virofem, mouse conc.

Used antibodies with expression pattern in spindle cell carcinoma (SPC) and inflammatory myofibroblastic tumor (IMT) as well as source and dilution. Staining started with antibodies in the upper part, followed by additional stainings, listed below. strong positive reaction (++), positive reaction (+), no reaction (NR), recommended for differential diagnosis (*).

ISSN: 1746-1596