Table 1 The panel of immunohistochemical reagents used
Antibody | Clone | Manufacturer | Pre-treatment | Dilution |
|---|---|---|---|---|
CK 7 | OV-TL 12/30 | Dako (Carpinteria, CA) | Pepsin for 10 minutes at 37°C | 1/4000 |
CK 20 | KS 20.8 | Novocastra (Vision Biosystem, Norwell, MA) | Pepsin for 10 minutes at 37°C | 1/50 |
Synaptophysin | 27G12 | Vector (Burlingame, CA) | HIER at pH 6.0 | 1/200 |
Chromogranin A | Polyclonal | Dako (Carpinteria, CA) | Pepsin for 10 minutes at 37°C | 1/200 |
CD56 | 123C3 | Zymed (Invitrogen, Carlsbad, CA) | HIER at pH 6.0 | 1/200 |
NSE | Polyclonal | Dako (Carpinteria, CA) | None | 1/250 |
EGFR | 31G7 | Zymed (Invitrogen, Carlsbad, CA) | Pepsin for 10 minutes at 37°C | 1/100 |
LMWK | Cam 5.2 | B.D. Bioscience (San Jose, CA) | Pepsin for 10 minutes at 37°C | 1/64 |
CK 19 | 6170 | Novocastra (Vision Biosystem, Norwell, MA) | Pepsin for 10 minutes at 37°C | 1/50 |
Pankeratin | AE1/AE3 | Dako (Carpinteria, CA) | Pepsin for 10 minutes at 37°C | 1/300 |
TTF-1 | 8G7G3/1 | Neomarker (Lab Vision, Fremont, CA) | HIER at pH 6.0 | 1/800 |
CA 125 | Ov185:1 | Novocastra (Vision Biosystem, Norwell, MA) | HIER at pH 6.0 | 1:100 |
CDX2 | Amt 28 | Vision Biosystem (Norwell, MA) | HIER at pH 8.0 | 1/50 |
mCEA | B80-1 | Biomeda (Foster City, CA) | None | 1/200 |