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Table 1 The panel of immunohistochemical reagents used

From: Primary colorectal small cell carcinoma: A clinicopathological and immunohistochemical study of 10 cases

Antibody Clone Manufacturer Pre-treatment Dilution
CK 7 OV-TL 12/30 Dako (Carpinteria, CA) Pepsin for 10 minutes at 37°C 1/4000
CK 20 KS 20.8 Novocastra (Vision Biosystem, Norwell, MA) Pepsin for 10 minutes at 37°C 1/50
Synaptophysin 27G12 Vector (Burlingame, CA) HIER at pH 6.0 1/200
Chromogranin A Polyclonal Dako (Carpinteria, CA) Pepsin for 10 minutes at 37°C 1/200
CD56 123C3 Zymed (Invitrogen, Carlsbad, CA) HIER at pH 6.0 1/200
NSE Polyclonal Dako (Carpinteria, CA) None 1/250
EGFR 31G7 Zymed (Invitrogen, Carlsbad, CA) Pepsin for 10 minutes at 37°C 1/100
LMWK Cam 5.2 B.D. Bioscience (San Jose, CA) Pepsin for 10 minutes at 37°C 1/64
CK 19 6170 Novocastra (Vision Biosystem, Norwell, MA) Pepsin for 10 minutes at 37°C 1/50
Pankeratin AE1/AE3 Dako (Carpinteria, CA) Pepsin for 10 minutes at 37°C 1/300
TTF-1 8G7G3/1 Neomarker (Lab Vision, Fremont, CA) HIER at pH 6.0 1/800
CA 125 Ov185:1 Novocastra (Vision Biosystem, Norwell, MA) HIER at pH 6.0 1:100
CDX2 Amt 28 Vision Biosystem (Norwell, MA) HIER at pH 8.0 1/50
mCEA B80-1 Biomeda (Foster City, CA) None 1/200
  1. HIER = Heat-induced epitope retrieval, in a Biocare decloaking chamber