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Figure 1 | Diagnostic Pathology

Figure 1

From: Development of automated brightfield double In Situ hybridization (BDISH) application for HER2 g ene and chromosome 17 centromere (CEN 17) for breast carcinomas and an assay performance comparison to manual dual color HER2 fluorescence In Situ hybridization (FISH)

Figure 1

Brightfield double in situ hybridization (BDISH) signal detection scheme with a sequential in situ hybridization method. HER2 gene signal was detected with a DNP-labeled nick translated DNA probe hybridization followed by silver signal detection system (silver acetate, hydroquinone, and H2O2 reaction). Then, chromosome 17 centromere (CEN 17) signal was detected with a DNP-labeled CEN 17 oligoprobe hybridization followed by fast red and naphthol phosphate reaction signal detection system.

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