Table 3 FISH panel and virtual karyotype results for 22 morphologically challenging renal tumors
Sample ID | Histologic Diagnosis | FISH | Virtual Karyotype | Virtual Karyotype Interpretation | Array Result vs. Final Diagnosis* | Outcome | Months Follow up |
|---|---|---|---|---|---|---|---|
MC01 | Low grade neoplasm, favor oncocytoma | Not done | -1, -14, -21 | Oncocytoma | Confirms | No evidence of tumor | 9 |
MC02 | Low grade neoplasm, favor oncocytoma | Not done | del(10)(p11.23-p14) | Oncocytoma | Confirms | Disease status Unknown | 1 |
MC09 | Eosinophilic epithelial tumor morphologically consistent with eosinophilic renal cell carcinoma | -1 (44%), -2 (52%), -7 (38%), and -17 (88%) | -1, -1, -2, -3, +5, UPD 6, +7, -9, -9, -10p, -10p, del(10)(q24.33-qter), -11, -11, +12, -13,-13, +15(q22.2-qter), +16p, -17p, -17p, -17p, -17q, -17q, -18, +19, -21, -21, -22, -22, -22 | [inferred tetrasomy] | Mixed pattern CRCC/CHRCC = Unclassified | Novel pattern | Local & regional lymph node recurrence 52 months after nephrectomy Alive at last F/U | 69 |
MC10 | Oncocytic renal neoplasm, favor carcinoma | -1 (51%), 2 failed, +7 (21%), +7+7 (8%), +17 (30%) and intermediate -17(28%) | -1, -14 | Oncocytoma | SNP diagnostic | No evidence of tumor | 30 |
MC11 | Renal cell carcinoma, clear cell type with focal granular (eosinophilic) morphology | CHRCC area: +2 (75%) with intermed -1, -7, -17; CRCC area: -1, -2, -17 (34%, 36%, 30%) | UPD(3)(p14.1-p13.2) | Clear Cell RCC | Confirms | No evidence of tumor | 33 |
MC12 | Eosinophilic variant of clear cell renal carcinoma with papillary features. | -2 (31%), -7 (37%), -17 (97%) | del(4)(p15.1-pter), +5(q21.3-qter), +9p(UPD), del(11)(q13.3-qter), +12, +16, del(17p), +17(q21.32-qter), +17(q21.32-qter), +20, -22 | Novel, not consistent with clear cell | Novel pattern | Never disease free. Deceased | 2 |
MC13 | Oncocytic renal cell carcinoma, most suggestive of eosinophilic variant of conventional clear cell carcinoma | -1 (97%), -2 (83%), -7 (70%), and -17 (97%) | UPD(3)(p12.2-p24.1) | Clear Cell RCC | Confirms | No evidence of tumor | 3 |
MC14 | Chromophobe renal cell carcinoma | -1 (48%), -2 (50%), -7 (56%), and -17 (47%) | No detectable chromosomal abnormalities | Oncocytoma | Discrepant | No evidence of tumor | 29 |
MC15 | Renal cell carcinoma with morphologic features consistent with eosinophilic variant of clear cell carcinoma | -2 (37%), -7 (45%), -17 intermediate (20%) | del(1)(p32.3-pter), del(3)(p12.2-pter) | Clear Cell RCC | Confirms | Never disease free. Deceased | 19 |
MC16 | Oncocytic renal epithelial neoplasm, favor chromophobe renal cell carcinoma with eosinophilic morphology | -1(48%), -2 (40%), -7 (36%), and -17 (42%) | -1p, -9q, +12, -18, -21 | Oncocytoma (novel) | Discrepant | No evidence of tumor | 30 |
MC18 | Low grade carcinoma with myxoid matrix and spindle and tubular architecture | -1 (85%), -2 (67%), -7 (37%), and -17 (73%) | -1, -4, -6, -8, -9, -13, -14, -15, -17, -22 | MTSCC | Confirms | No evidence of tumor | 19 |
MC19 | Renal cell carcinoma with morphologic features of a chromophobe renal cell carcinoma. Multiple other tumors (2 papillary, 2 clear cell) | -1 (38%), -2 (42%), -7 (33%). 17 failed. | -3p, +3q, +7, del(9)(p13.2-p22.3) | Clear Cell RCC | Confirms | No evidence of tumor | 21 |
MC20 | Eosinophilic renal cell carcinoma | Intermed -1 (25%), -2 (52%), -7 (42%), -17 | No detectable chromosomal abnormalities | Oncocytoma | Discrepant | No evidence of tumor | 24 |
MC21 | Oncocytic renal epithelial neoplasm | -1 (92%), -17 (37%), +7 (51%) | -1,+7, +9p, -9q | Oncocytoma (novel) | SNP diagnostic | No evidence of tumor | 20 |
MC22 | Renal cell carcinoma, unclassified | -1 (45%), 12 (37%), -17 (50%); intermed -7 (22%) | +7, +16 | Papillary RCC | SNP diagnostic | No evidence of tumor | 20 |
MC23 | Eosinophilic renal cell carcinoma | -1 (49%), -2 (53%), -7 (49%), and -17 (74%) | No detectable chromosomal abnormalities | Oncocytoma | Discrepant | No evidence of tumor | 22 |
MC24 | Papillary renal cell carcinoma, type 2 | -1 (48%), intermed -2 (23%), -7 (26%) | +7, +12, +17 | Papillary RCC | Confirms | No evidence of tumor | 27 |
MC26 | Renal clear cell carcinoma | -2 intermed (23%). +7 (52%), -17 (43%) | -3p, unable to interpret other changes | Clear Cell RCC | Confirms | No evidence of tumor | 1 |
MC28 | Renal oncocytoma | -1 (37%), -2 (38%), -7 (30%), and -17 (55%) | No detectable chromosomal abnormalities | Oncocytoma | Confirms | No evidence of tumor | 68 |
MC29 | Papillary renal cell carcinoma, type II | -2 intermediate (20%),+7 (30%), +7+7 (18%) | +7, +16q (high normal contamination) | Papillary RCC | Confirms | No evidence of tumor | 16 |
MC30 | Renal oncocytoma | 1 -(41%), 2 failed, -7(34%), -17(65%) | No detectable chromosomal abnormalities | Oncocytoma | Confirms | No evidence of tumor | 71 |
MC31 | Renal epithelial oncocytic neoplasm with features of an oncocytoma | -1 (67%), -2 (63%), -7 (42%), and -17 (40%) | No detectable chromosomal abnormalities | Oncocytoma | Confirms | No evidence of tumor | 19 |