Tissue microarrays: one size does not fit all

Diagnostic Pathology

Table 3 Whole tissue section intra-reader agreement and agreement between the second whole tissue section and TMA

	B7-H1	B7-H3	Survivin	Ki-67	CAIX*	IMP3
	Kappa	CCC	CCC	CCC	CCC	CCC
Whole section	0.78	0.97	0.71	0.90	0.94	0.96
Cores
1	0.21 (0.00 - 0.65)	0.80 (0.73 - 0.87)	0.35 (0.06 - 0.55)	0.63 (0.44 - 0.82)	0.85 (0.73 - 0.94)	0.73 (0.02 - 0.87)
2	0.21 (0.00 - 0.65)	0.81 (0.74 - 0.87)	0.40 (0.12 - 0.57)	0.72 (0.49 - 0.84)	0.82 (0.73 - 0.92)	0.79 (0.20 - 0.85)
3	0.38 (0.00 - 0.65)	0.83 (0.76 - 0.86)	0.43 (0.24 - 0.62)	0.76 (0.62 - 0.86)	0.80 (0.72 - 0.89)	0.81 (0.49 - 0.85)
4	0.38 (0.00 - 0.65)	0.83 (0.77 - 0.85)	0.44 (0.33 - 0.58)	0.79 (0.64 - 0.85)	0.78 (0.71 - 0.87)	0.82 (0.61 - 0.85)
5	0.52 (0.00 - 0.65)	0.83 (0.80 - 0.85)	0.45 (0.36 - 0.57)	0.81 (0.71 - 0.86)	0.77 (0.72 - 0.84)	0.82 (0.77 - 0.85)
6	0.52 (0.00 - 0.65)	0.84 (0.81 - 0.84)	0.45 (0.37 - 0.57)	0.81 (0.71 - 0.85)	0.76 (0.71 - 0.82)	0.82 (0.77 - 0.85)
7	0.65 (0.00 - 0.65)	0.84 (0.81 - 0.84)	0.46 (0.40 - 0.52)	0.82 (0.73 - 0.85)	0.75 (0.72 - 0.82)	0.82 (0.77 - 0.85)
8	0.65 (0.38 - 0.65)	0.84 (0.83 - 0.84)	0.46 (0.41 - 0.51)	0.82 (0.76 - 0.84)	0.74 (0.72 - 0.81)	0.82 (0.79 - 0.85)
9	0.65 (0.52 - 0.65)	0.84 (0.83 - 0.84)	0.46 (0.43 - 0.48)	0.82 (0.79 - 0.84)	0.73 (0.72 - 0.79)	0.82 (0.80 - 0.84)
10	0.65 (0.65 - 0.65)	0.84 (0.84 - 0.84)	0.46 (0.46 - 0.46)	0.82 (0.82 - 0.82)	0.73 (0.73 - 0.73)	0.82 (0.82 - 0.82)

*CAIX was analyzed using the minimum value across cores. All other biomarkers were analyzed using the maximum value across cores.
The kappa statistic is reported for B7-H1, whereas the concordance correlation coefficient (CCC) is reported for B7-H3, survivin, Ki-67, CAIX, and IMP3; the median (minimum, maximum) statistic from the 500 bootstrap samples is provided.

ISSN: 1746-1596